| GLYCO-GENE CHIP
Investigator's Responsibility
|
RNA Sample Requirements:
|
1. Ideally, 2 micrograms of total RNA should be provided at a concentration of 0.5- 1.0
microgram per microliter in water (this amount allows for sample quality control, labeling, and any necessary re-preps). For most studies, 0.5 to 1.0 micrograms will be sufficient for labeling. However, as little as 10 - 100 nanograms may be sufficient with an alternate amplification protocol. Please see the
Core E protocols or contact the Director or contact the Director for further information regarding RNA samples that are less than 2 micrograms.
2. RNA may be extracted using a variety of methods as long as the final product is not degraded and is free of RNAses and traces of organic solvents used in the extraction process. We recommend RNA preparation kits from Qiagen (RNeasy Mini Kit) or an equivalent kit available from any number of vendors. A minimum of 2 ug total RNA is requested in most cases. See QIAGEN RNeasyMiniKit http://glycomics.scripps.edu/coreeRNeasy.pdf for details of extraction protocol.
Note: Purified RNA should be sent.
|
|
Experimental Design: |
Because it is not practical to develop one
experimental design that is appropriate for all microarray
experiments, several factors should be considered when designing
microarray experiments for measuring gene expression changes in
biological systems. Please see the
PDF file for more information.
|
|
Shipping Instructions: |
1. The easiest and safest way to ship total RNA samples is in RNAstable (from Biomatrica., http://www.biocompare.com/newtech/2320/RNAstable.html ). We have tested this product and found it to be excellent for storage and shipping of RNA at room temperature when manufacturer instructions are carefully followed. After RNA is dried down in RNAstable, the sample must be enclosed in an airtight container or pouch with desiccant added to protect the RNA from ambient humidity. Your sample can be shipped at room temperature and for a much lower cost than shipping on dry ice.
2. If you prefer to ship on dry ice, please pack with adequate dry ice to keep RNA frozen for several days. International shipping routinely takes a minimum of two day and any delays in shipping will result in possible loss of sample integrity if an adequate amount of dry ice is not used. All shipments on dry ice should be mailed on a Monday or Tuesday to avoid having delayed shipments arrive on the weekend. Note: Please no weekend deliveries.
3. Core E should be notified by email when shipments are sent and provided with tracking information to assist us in searching for delayed shipments. Please refer to shipping address and contact information below.
Please note that the Core E pipeline is approaching full capacity. Therefore, samples will be analyzed on a first-come, first-served basis and must be received by Core E within the next few months (by early January 2011) in order to be analyzed. Investigators with approved Resource Requests should notify Core E if they no longer plan to submit samples; contact information is provided below.
Address shipments to:
| Steven Head/Gilberto Hernandez |
| Mail Code SP-256 |
| DNA Array Core Facility |
| 10550 North Torrey Pines Road |
| La Jolla, CA 92037 |
| Contact Information: |
| Steven Head (858) 784-2263 shead@scripps.edu |
| Gilberto Hernandez (858) 784-2263 gilberto@scripps.edu |
|
Core's Responsibility
Request Glyco-gene Chip Analysis or Glyco-gene Chips
Shipping Address
|