DC-SIGN - Revision history

Kurt Drickamer: /* Biosynthesis of ligands */

2011-04-15T07:43:38Z

Biosynthesis of ligands

2011-04-15T07:41:54Z

Glycan profiling

2011-04-14T16:15:49Z

Glycan profiling */

2011-03-21T13:58:33Z

2011-03-18T21:47:22Z

Related GBPs

2011-03-18T21:29:44Z

2011-03-17T19:03:44Z

Biosynthesis of ligands

2011-03-16T19:31:18Z

Glycogene microarray

2011-03-16T19:28:23Z

Structure

2011-03-16T19:26:13Z

Structure

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 === Biosynthesis of ligands ===
 <b>Glycans on viruses</b><br>
 High mannose oligosaccharides on viral envelope proteins that are ligands for DC-SIGN result from incomplete processing of glycans in the pathway for biosynthesis of complex N-linked glycans ([http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/geMolecule.jsp?slideNumber=default GT Database]).

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 <br>
 T-Cells, which would be the most relevant interacting partner, contain substantial levels of Le<sup>X</sup> in humans.
 === Glycogene microarray ===

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 === Glycan profiling ===
 === Glycogene microarray ===
 Probes for human DC-SIGN have been included in all versions of the CFG glycogene chip.

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 <br><br>
 <b>Glycans on parasites</b><br>
-<br>
+Some data give insight in the biosynthesis of DC-SIGN ligands in parasitic helminths. In <i>Schistosoma mansoni</i> <ref>DeBose-Boyd R, Nyame AK, Cummings RD. 1996. <i>Schistosoma mansoni</i>: characterization of an &alpha;1-3 fucosyltransferase in adult parasites. Exp Parasitol. 82: 1-10</ref><ref>Marques Jr ET Jr, Ichikawa Y, Strand M, August JT, Hart GW, Schnaar RL. 2001. Fucosyltransferases in ''Schistosoma mansoni'' development. Glycobiology 11: 249-59</ref> and ''Haemonchus contortus'' <ref>DeBose-Boyd RA, Nyame AK, Jasmer DP, Cummings RD. 1998. The ruminant parasite ''Haemonchus contortus'' expresses an α1,3-fucosyltransferase capable of synthesizing the Lewis x and sialyl Lewis x antigens. Glycoconjugate J. 15: 789-98</ref> &alpha;1,3-fucosyltransferases have been identified, which may be involved in generation of Gal&beta;1-4(Fuc&alpha;1-3)GlcNAc-R (Lewis X), and/or GalNAc&beta;1-4(Fuc&alpha;1-3)GlcNAc-R (LDNF). Combined transcriptome (putative glycosyltransferase genes) and glycome analyses of Schistosoma revealed that female schistosomes synthesize preferably terminal LacNAc and Lewis X, whereas male worms synthesize more LDN/LDNF antigens <ref>Hokke CH, Fitzpatrick JM and Hoffmann KF. 2007.  Integrating transcriptome, proteome and glycome analyses of Schistosoma biology.  Trends in Parasitology 23: 165-174</ref>.
 === Structure ===

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 == Related GBPs ==
-Other dendritic cell lectins include langerin, DCIR, and DCAR. Paralogs on other cells include DC-SIGNR.
+Other dendritic cell lectins include langerin [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=langerin&maxresults=20 (CFG data)], DCIR [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=DCIR&maxresults=20 (CFG data)], and DCAR. Paralogs on other cells include DC-SIGNR [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=DC-SIGNR&maxresults=20 (CFG data)].
 == References ==

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 === Glycan array ===
-Glycan array analysis <ref name="Guo 2004"/><ref name="Liempt 2006"/>and synthetic oligosaccharides were used to elucidate  DC-SIGN  [http://www.functionalglycomics.org/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_52_06122003 glycan-binding specificity] and analyze the mechanism of specific glycan binding. See all glycan array results for [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=dc-sign&cat=coreh DC-SIGN here].
+Glycan array analysis <ref name="Guo 2004"/><ref name="Liempt 2006"/>and synthetic oligosaccharides were used to elucidate  DC-SIGN  [http://www.functionalglycomics.org/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_52_06122003 glycan-binding specificity] and analyze the mechanism of specific glycan binding. See all glycan array results for [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=dc-sign&cat=coreh DC-SIGN here]. See glycan array screening results for these related GBPs: [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=langerin&cat=coreh langerin,] [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=DCIR&cat=coreh DCIR,] and [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=DC-SIGNR&cat=coreh DC-SIGNR.]
 == Related GBPs ==

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 <br>
 === Glycogene microarray ===
 === Knockout mouse lines ===

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 <br>
 === Structure ===
-[[image:DC-SIGN_3.jpg]]<br>
+[[image:DC-SIGN_4.jpg]]<br>
 Crystal structures of the CRD of DC-SIGN bound to a mannose-containing oligosaccharide and lacto-N-fucopentaose have been analyzed.<ref name="Feinberg 2001"/><ref name="Guo 2004"/> The first structure can be used to model the binding of the outer portion of a high mannose oligosaccharide and the second structure shows how the Lewis<sup>x</sup> trisaccharide fits into the binding site. The high mannose oligosaccharide makes extensive interactions in an extended binding site, while the more rigid Lewis<sup>x</sup> oligosaccharide binds primarily through the fucose residues, with some additional stabilizing interactions with the galactose. The overall structure of the tetrameric extracellular domain has been deduced from crystal structures of the repeats in the neck domain of the related protein DC-SIGNR (L-SIGN)<ref name="Feinberg 2009">Feinberg, H, Tso, CKW, Taylor, ME, Drickamer, K and Weis, WI. 2009. Segmented helical structure of the neck region of the glycan-binding receptor DC-SIGNR. J Mol Biol 394:613-620</ref> and oligomeric C-terminal fragments of DC-SIGN that contain the CRD<ref name="Feinberg 2005">Feinberg, H, Guo, Y, Mitchell, DA, Drickamer, K and Weis, WI. 2005. Extended neck regions stabilze tetramers of the receptors DC-SIGN and DC-SIGNR. J Biol Chem 280:1327-1335</ref>.
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