F17G/GafD - Revision history

Kurt Drickamer: /* Cellular expression of GBP and ligands */

2011-10-29T11:42:04Z

Cellular expression of GBP and ligands

2011-10-29T11:41:24Z

Biosynthesis of ligands

2011-10-29T11:40:27Z

Biosynthesis of ligands

2011-04-16T09:36:22Z

Structure

2011-03-30T23:21:24Z

Glycogene microarray

2011-03-28T19:09:38Z

Glycogene microarray

2011-03-21T18:54:25Z

Related GBPs

2011-03-21T18:51:06Z

Knockout mouse lines

2010-09-20T22:22:30Z

Biological roles of GBP-ligand interaction

2010-08-06T17:40:35Z

Progress toward understanding this GBP paradigm

@@ Line 24: / Line 24: @@
 F17G adhesins are expressed on enterotoxigenic E. coli infecting neonatal lambs, calves, and goat kids.
 The F17G ligand GlcNAcb1,3Gal occurs universally but mostly internally in the sequence of poly-lactosaminyl glycans and blood group antigens.
-These glycan structures are widely expressed on mamalian cell surfaces .
+These glycan structures are widely expressed on mamalian cell surfaces.
 === Biosynthesis of ligands ===

@@ Line 33: / Line 33: @@
 [http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_562&sideMenu=true&pageType=general UDP-GlcNAc:&beta;Gal &beta;1-3 GlcNAc transferase 7].
 <br>
 === Structure ===

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 === Biosynthesis of ligands ===
 <br>
 F17-fimbriated E. coli predominantly colonize neonatal animals, but also are a major causal agent (55%) of mastitis in bovines <ref>Lipman, L.J.A., de Nijs A., Gaastra W.. Isolation and identification of fimbriae and toxin production by Escherichia coli strains from cows with clinical mastitis, Vet. Microbiology 47 (1995) p. 1-7 </ref>.  Congruent with the glycans recognized by F17G on the printed array versions 2.1 and 4.1, the N-acetyl glucosamine residue of GlcNAcb1,3Gal may be unsubstituted at the early life stage of calves, that are at the same time protected from bacterial infections by glycans secreted in the cow's milk.

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 === Structure ===
 The F17G adhesin is a two-domain adhesin (TDA) located at the F17 fimbrial tip. The determination of  the crystal structure of the F17G lectin domain led to the discovery of the variable immunoglobulin-like structure
 as a paradigm for bacterial fimbrial TDAs <ref name=" Buts, L2003">
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 <br>
-[[File:F17G_Igfold.jpg]]
+[[File:F17G_Igfold_v3.jpg‎]]
 <br>

@@ Line 51: / Line 51: @@
 <br>
 === Glycogene microarray ===
-Not applicable, as the microarrays only contain probes for mouse and human glycogenes.
+F17G/GafD is not represented on the CFG microarrays, which only contain probes for mouse and human glycogenes.
 <br>

@@ Line 61: / Line 61: @@
 == Related GBPs ==
-FedF, CfaE, FimH, PapG, CupB6
+FedF [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=FedF&maxresults=20 (CFG data)], CfaE [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=CfaE&maxresults=20 (CFG data)], FimH [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=FimH&maxresults=20 (CFG data)], PapG, CupB6 [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=CupB6&maxresults=20 (CFG data)].
 The specificity of some of the other fimbrial tip adhesins was determined by CFG glycan array analysis ([http://www.functionalglycomics.org/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_2358 ''P. gingivalis'' fimbriae], [http://www.functionalglycomics.org/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_PA_v2_178_11182005 ''E. coli'' FedF adhesin], [http://www.functionalglycomics.org/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_1106 ''E. coli'' CfaE adhesin from CFA/I pili])

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 <br>
 === Knockout mouse lines ===
 === Glycan array ===
 F17G adhesins have been screened for their glycan specificity (click [http://www.functionalglycomics.org/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_PA_v2_177_11182005 here]). To see all glycan array results for F17G adhesin, click [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=F17G&cat=coreh here].

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 === Biological roles of GBP-ligand interaction ===
-The F17G fimbrial lectin enhances intestinal colonization in the early life of ruminants. The long and flexible F17 fimbriae can penetrate deep between intestinal microvilli, where the fimbrial tip adhesin finds its glycan receptors. The subsequent secretion of heat stable and heat lable toxins can lead to severe diarhea.
+The F17G fimbrial lectin enhances intestinal colonization in the early life of ruminants. The long and flexible F17 fimbriae can penetrate deep between intestinal microvilli, where the fimbrial tip adhesin finds its glycan receptors. The subsequent secretion of heat stable and heat labile toxins can lead to severe diarrhea.
 == CFG resources used in investigations ==

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 == Progress toward understanding this GBP paradigm ==
+This section documents what is currently known about F17G/GafD, its carbohydrate ligand(s), and how they interact to mediate cell communication.
 === Carbohydrate ligands ===
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 === Cellular expression of GBP and ligands ===
 F17G adhesins are expressed on enterotoxigenic E. coli infecting neonatal lambs, calves, and goat kids.
 The F17G ligand GlcNAcb1,3Gal occurs universally but mostly internally in the sequence of poly-lactosaminyl glycans and blood group antigens.
 These glycan structures are widely expressed on mamalian cell surfaces .