Parvovirus Minute Virus of Mice (MVM) - Revision history

Kurt Drickamer: /* Biological roles of GBP-ligand interaction */

2011-04-09T11:57:23Z

Biological roles of GBP-ligand interaction

Kurt Drickamer: /* Biosynthesis of ligands */

2011-04-09T11:50:16Z

Biosynthesis of ligands

Carole Weaver: /* Glycogene microarray */

2011-03-30T23:24:36Z

Glycogene microarray

Carole Weaver: /* Related GBPs */

2011-03-21T20:05:03Z

Related GBPs

Anna Crie: /* Structure */

2010-08-02T21:57:41Z

Structure

Anna Crie: /* Structure */

2010-08-02T21:51:03Z

Structure

Anna Crie: /* Structure */

2010-08-02T21:11:12Z

Structure

Anna Crie: /* Structure */

2010-08-01T22:26:21Z

Structure

Anna Crie: /* Structure */

2010-08-01T22:24:01Z

Structure

Anna Crie: /* Structure */

2010-08-01T22:23:23Z

Structure

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 === Biological roles of GBP-ligand interaction ===
-Glycan recognition by members of the Parvoviridae family dictates tissue tropism and is involved in tissue tropism and pathogenicity differences between highly homologous strains for several pathogenic members.
+Interaction with cell surface sialic acid, in the context of a glycoprotein, is an essential first step in cellular recognition and tropism by MVM<ref name="Cotmore 1987"/><ref name="Lopez-Bueno 2006"/>. Glycan recognition by members of the Parvoviridae family dictates tissue tropism and is involved in tissue tropism and pathogenicity differences between highly homologous strains for several pathogenic members.
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 === Biosynthesis of ligands ===
-Sialylated glycoproteins recognized by MVM are synthesized by host cells.
+Sialylated glycoproteins recognized by MVM are synthesized by host cells. The enzyme required for biosynthesis of the type 2 poly <i>N</i>-acetyllactosamine chains and modification with sialic acid or with sialic acid and fucose, creating the Lewis<sup>x</sup> epitope, have been defined ([http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/geMolecule.jsp?slideNumber=slide7 poly <i>N</i>-acetyllactosamine extension biosynthesis]). Gangliosides, sialylated oligosaccharides, are synthesized by the host by well defined pathways ([http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/geMolecule.jsp?slideNumber=slide9 Glucosylceramide biosynthesis]).
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 === Glycogene microarray ===
-N/A
+MVM is not represented on the CFG microarrays, which only contain probes for mouse and human glycogenes.
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@@ Line 56: / Line 56: @@
 == Related GBPs ==
-Other parvovirus GBPs have been studied by the CFG, including H1 (rat parvovirus) and several AAV serotypes: AAV1, AAV2, AAV4-AAV9<ref name="Wu 2006">Wu Z, E. Miller E, M. Agbandje-McKenna, R. J. Samulski. 2006. {alpha}2,3 and {alpha}2,6 N Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated Virus Types 1 and 6. J. Virol., 80:9093-9103. </ref> (and unpublished data).  Glycan array analyses have been used to identify glycans recognized by the capsids of these viruses, which includes terminal sialic acid containing oligosaccharides as well as those which terminate in galatose <ref name="Wu 2006"/>(and unpublished data).
+Other parvovirus GBPs have been studied by the CFG, including H1 (rat parvovirus) and several AAV serotypes: AAV1 [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=AAV1&maxresults=20 (CFG data)], AAV2, AAV4-AAV9<ref name="Wu 2006">Wu Z, E. Miller E, M. Agbandje-McKenna, R. J. Samulski. 2006. {alpha}2,3 and {alpha}2,6 N Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated Virus Types 1 and 6. J. Virol., 80:9093-9103. </ref> (and unpublished data; CFG data: [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=AAV5&maxresults=20 AAV5,] [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=AAV6&maxresults=20 AAV6,] [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=AAV7&cat=coreh AAV7,] [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=AAV8&maxresults=20 AAV8,] [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=AAV9&maxresults=20 AAV9.]) Glycan array analyses have been used to identify glycans recognized by the capsids of these viruses, which includes terminal sialic acid containing oligosaccharides as well as those which terminate in galatose <ref name="Wu 2006"/>(and unpublished data).
 == References ==

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 === Structure ===
 MVM is assembled from three VPs, VP1, VP2, and VP3, with the entire sequence of the smallest VP3 contained within VP2 which is in turn contained with VP1; VP1 has a unique N-terminal PLA2 region.  VP3, which is only generated from VP2 following genome packaging, forms the majority of the capsid protein content, at ~90%, and contains the receptor recognition site.  Significantly, this receptor-recognition site is only created in the assembled capsid with amino acid contributions from icosahedral symmetry related VPs<ref name="Kontou 2005"/>.
-<br>
+<br>[[File:MVMMonoCapsid.jpg]]
-[[File:MVMMonoCapsid.jpg]]
+<br><br>
 The glycan structures below have been observed to interact with MVM capsids using the CFG glycan array
 <br>

@@ Line 26: / Line 26: @@
 MVM is assembled from three VPs, VP1, VP2, and VP3, with the entire sequence of the smallest VP3 contained within VP2 which is in turn contained with VP1; VP1 has a unique N-terminal PLA2 region.  VP3, which is only generated from VP2 following genome packaging, forms the majority of the capsid protein content, at ~90%, and contains the receptor recognition site.  Significantly, this receptor-recognition site is only created in the assembled capsid with amino acid contributions from icosahedral symmetry related VPs<ref name="Kontou 2005"/>.
 <br>
-The glycan structures below have been observe to interact with MVM capsids using the CFG glycan array
+The glycan structures below have been observed to interact with MVM capsids using the CFG glycan array
 <br>
 [[File:structuresGD3SiaLN.jpg]]

@@ Line 28: / Line 28: @@
 The glycan structures below have been observe to interact with MVM capsids using the CFG glycan array
 <br>
- [[Image:structuresGD3SiaLN.jpg]]
 <br>