Siglec-8 - Revision history

Carole Weaver: /* Structure */

2011-09-12T22:18:49Z

Structure

Carole Weaver: /* Glycogene microarray */

2011-03-30T23:32:17Z

Glycogene microarray

Kurt Drickamer: /* Biosynthesis of ligands */

2011-03-20T13:10:31Z

Biosynthesis of ligands

Carole Weaver: /* Related GBPs */

2011-03-18T22:24:11Z

Related GBPs

Carole Weaver at 18:33, 20 September 2010

2010-09-20T18:33:49Z

Carole Weaver: /* Progress toward understanding this GBP paradigm */

2010-08-06T17:29:55Z

Progress toward understanding this GBP paradigm

Jim Paulson: /* Glycan array */

2010-07-20T13:54:38Z

Glycan array

Jim Paulson: /* Glycogene microarray */

2010-07-20T13:50:56Z

Glycogene microarray

Jim Paulson: /* Glycan profiling */

2010-07-20T12:59:47Z

Glycan profiling

Carole Weaver at 21:45, 6 July 2010

2010-07-06T21:45:00Z

@@ Line 37: / Line 37: @@
 [[File:Siglec8 SiglecF.jpg]]
 <br>
 === Biological roles of GBP-ligand interaction ===

@@ Line 54: / Line 54: @@
 === Glycogene microarray ===
-Analysis has been conducted on glycosyltransferase expression using the glycogene microarray for [http://www.functionalglycomics.org/static/coreE/flash/search.swf?maExpKey=100393 murine eosinophils] that relates to the enzymes required for expression of cis ligands of Siglec-F on these cells.
+Analysis has been conducted on glycosyltransferase expression using the glycogene microarray for [http://www.functionalglycomics.org/static/coreE/flash/search.swf?maExpKey=100393 murine eosinophils] that relates to the enzymes required for expression of cis ligands of Siglec-F on these cells. Probes for human Siglec-8 and mouse Siglec-F have been included on all four versions of the CFG glycogene microarray.
 === Knockout mouse lines ===

@@ Line 29: / Line 29: @@
 <br>
 === Biosynthesis of ligands ===
-Constitutive expression in airway epithelium of the putative &alpha;2,3-linked sialylated glycoprotein ligand for Siglec-F requires the enzyme St3Gal3 (see above). <br>
+Constitutive expression in airway epithelium of the putative &alpha;2,3-linked sialylated glycoprotein ligand for Siglec-F requires the enzyme St3Gal3 which has been characterized in
 === Structure ===
 [[File:Siglec8 SiglecF.jpg]]

@@ Line 60: / Line 60: @@
 == Related GBPs ==
-hSiglec-3 (CD33), Siglec-5, Siglec-6, Siglec, 7, Siglec-9, Siglec-10, Siglec-11, Siglec-F, Siglec-E, Siglec-G
+hSiglec-3 (CD33), Siglec-5, Siglec-6 [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=siglec-6&maxresults=20 (CFG data)], Siglec-7 [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=siglec-7&maxresults=20 (CFG data)], Siglec-9 [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=siglec-9&maxresults=20 (CFG data)], Siglec-10 [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=siglec-10&maxresults=20 (CFG data)], Siglec-11, Siglec-F [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=siglec-F&maxresults=20 (CFG data)], Siglec-E [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=siglec-E&maxresults=20 (CFG data)], Siglec-G [http://www.functionalglycomics.org/glycomics/search/jsp/landing.jsp?query=siglec-G&maxresults=20 (CFG data)].
 == References ==

@@ Line 29: / Line 29: @@
 <br>
 === Biosynthesis of ligands ===
-<br>
+Constitutive expression in airway epithelium of the putative &alpha;2,3-linked sialylated glycoprotein ligand for Siglec-F requires the enzyme St3Gal3 (see above). <br>
 === Structure ===
 [[File:Siglec8 SiglecF.jpg]]

@@ Line 13: / Line 13: @@
 == Progress toward understanding this GBP paradigm ==
+This section documents what is currently known about Siglec-8, its carbohydrate ligand(s), and how they interact to mediate cell communication. Further information about Siglec-8 can be found in its [http://www.functionalglycomics.org/glycomics/molecule/jsp/viewGbpMolecule.jsp?gbpId=cbp_hum_Itlect_00146&sideMenu=no GBP Molecule Page] in the CFG database.
 === Carbohydrate ligands ===
 The high affinity ligand for Siglec-8 has been deduced from glycan microarray screening on the CFG microarray (click [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_49_09152004 here] and [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_19_02202004 here]) to be NeuAcα2-3(6-SO3)Galβ1-4(Fucα1-3)GlcNAc [6'Su-SLeX] <ref name=" Bochner 4307 ">

@@ Line 57: / Line 57: @@
 === Glycan array ===
-The discovery of the ligand for Siglec-8 (click [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_49_09152004 here] and [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_19_02202004 here]) and its murine paralog, Siglec-F (click [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_47_10132004 here] and [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_58_07262004 here]), was made by investigator-initiated resource requests for glycan array analysis and carbohydrate compounds. To see all glycan array results for Siglec-8, click [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=siglec-8&cat=coreh here].
+The discovery of the [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_49_09152004 ligand for Siglec-8] [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_19_02202004] and its murine paralog, [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_47_10132004 Siglec-F ] [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_58_07262004], was made by investigator-initiated resource requests for glycan array analysis and carbohydrate compounds. To see all glycan array results for Siglec-8, click [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=siglec-8&cat=coreh here].
 == Related GBPs ==

@@ Line 47: / Line 47: @@
 === Glycan profiling ===
-Glycan structure analysis has been conducted by the CFG for human (click [http://www.functionalglycomics.org/glycomics/common/jsp/samples/searchSample.jsp?5=Human&15=&10=&9=Eosinophils+&operation=refine&templateKey=2&12=CellType&submit=submit here]) and mouse (click [http://www.functionalglycomics.org/glycomics/common/jsp/samples/searchSample.jsp?5=Mouse&15=&10=&9=Eosinophils+&operation=refine&templateKey=2&12=CellType&submit=submit here]) eosinophils.
+Glycan structure analysis has been conducted by the CFG for [http://www.functionalglycomics.org/glycomics/common/jsp/samples/searchSample.jsp?5=Human&15=&10=&9=Eosinophils+&operation=refine&templateKey=2&12=CellType&submit=submit human ] and [http://www.functionalglycomics.org/glycomics/common/jsp/samples/searchSample.jsp?5=Mouse&15=&10=&9=Eosinophils+&operation=refine&templateKey=2&12=CellType&submit=submit mouse] eosinophils.
 === Glycogene microarray ===

@@ Line 15: / Line 15: @@
 === Carbohydrate ligands ===
-The high affinity ligand for Siglec-8 has been deduced from glycan microarray screening on the CFG microarray[http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_49_09152004][http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_19_02202004] to be NeuAcα2-3(6-SO3)Galβ1-4(Fucα1-3)GlcNAc [6'Su-SLeX] <ref name=" Bochner 4307 ">
+The high affinity ligand for Siglec-8 has been deduced from glycan microarray screening on the CFG microarray (click [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_49_09152004 here] and [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_19_02202004 here]) to be NeuAcα2-3(6-SO3)Galβ1-4(Fucα1-3)GlcNAc [6'Su-SLeX] <ref name=" Bochner 4307 ">
 </ref><ref name=" Tateno 1125"></ref>
@@ Line 25: / Line 25: @@
 === Cellular expression of GBP and ligands ===
-Siglec-8 is expressed in human eosinophils, mast cells, and basophils (weakly). <ref name=" Kikly 2009"></ref>
+Siglec-8 is expressed in human eosinophils and mast cells, and weakly in basophils. <ref name=" Kikly 2009"></ref>
 <ref name=" Floyd H  2000">Floyd H, Ni J, Cornish AL, Zeng Z, Liu D, Carter KC, Steel J, Crocker PR. Siglec-8: a novel eosinophil-specific member of the immunoglobulin superfamily. J Biol Chem 2000; 275:861-6.</ref><ref>Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS. Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells. Allergy 2006; 61:769-76</ref>
 <br>
@@ Line 47: / Line 47: @@
 === Glycan profiling ===
-Glycan structure analysis has been conducted by the CFG for human[http://www.functionalglycomics.org/glycomics/common/jsp/samples/searchSample.jsp?5=Human&15=&10=&9=Eosinophils+&operation=refine&templateKey=2&12=CellType&submit=submit] and mouse[http://www.functionalglycomics.org/glycomics/common/jsp/samples/searchSample.jsp?5=Mouse&15=&10=&9=Eosinophils+&operation=refine&templateKey=2&12=CellType&submit=submit] eosinophils.
+Glycan structure analysis has been conducted by the CFG for human (click [http://www.functionalglycomics.org/glycomics/common/jsp/samples/searchSample.jsp?5=Human&15=&10=&9=Eosinophils+&operation=refine&templateKey=2&12=CellType&submit=submit here]) and mouse (click [http://www.functionalglycomics.org/glycomics/common/jsp/samples/searchSample.jsp?5=Mouse&15=&10=&9=Eosinophils+&operation=refine&templateKey=2&12=CellType&submit=submit here]) eosinophils.
 === Glycogene microarray ===
-Analysis has been conducted on glycosyltransferase expression using the glycogene microarray for murine eosinophils that relates to the enzymes required for expression of cis ligands of Siglec-F on these cells[http://www.functionalglycomics.org/static/coreE/flash/search.swf?maExpKey=100393].
+Analysis has been conducted on glycosyltransferase expression using the glycogene microarray for murine eosinophils that relates to the enzymes required for expression of cis ligands of Siglec-F on these cells (click [http://www.functionalglycomics.org/static/coreE/flash/search.swf?maExpKey=100393 here]).
 === Knockout mouse lines ===
@@ Line 57: / Line 57: @@
 === Glycan array ===
-The discovery of the ligand for Siglec-8[http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_49_09152004][http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_19_02202004] and its murine paralog, Siglec-F[http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_47_10132004][http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_58_07262004], was made by investigator-initiated resource requests for glycan array analysis and carbohydrate compounds. To see all glycan array results for Siglec-8, click [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=siglec-8&cat=coreh here].
+The discovery of the ligand for Siglec-8 (click [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_49_09152004 here] and [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_19_02202004 here]) and its murine paralog, Siglec-F (click [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v3_47_10132004 here] and [http://www.functionalglycomics.org:80/glycomics/HServlet?operation=view&sideMenu=no&psId=primscreen_GLYCAN_v2_58_07262004 here]), was made by investigator-initiated resource requests for glycan array analysis and carbohydrate compounds. To see all glycan array results for Siglec-8, click [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=siglec-8&cat=coreh here].
 == Related GBPs ==