P-Selectin
The three selectins (P-selectin, L-selectin, and E-selectin) have related and sometimes overlapping functions in cell adhesion and mediate some of the best characterized glycan-dependent cell adhesion events. Of these three C-type lectins, the target ligand of P-selectin, P-selectin glycoprotein ligand 1 (PSGL-1), is the best understood. Thus, P-selectin is used here to represent all three selectins.
CFG Participating Investigators contributing to the understanding of this paradigm
Selectin research was already at a relatively mature stage when the CFG began. Early PI work included structural studies, extensive analysis of leukocyte adhesion to endothelia in vivo, and characterization of knockout mice to demonstrate physiological function. In addition to submitting samples for glycan array analysis, PIs have been involved in analyzing selectin expression under different conditions, including in knockout mice lacking enzymes for making target ligands.
- PIs working on P-selectin include: Hans-Peter Altevogt, Bruce Bochner, Pi-Wan Cheng, Richard Cummings, Robert Fuhlbrigge, Minoru Fukuda, Geoff Kansas, Klaus Ley, John Lowe, Rodger McEver, Steve Rosen, Ron Schnaar, Karen Snapp, Lloyd Stoolman, Martin Wild, Hermann Ziltener
- Non-PIs who have used CFG resources to study P-selectin include: Roland Contreras, Leonard Seymour
Progress toward understanding this GBP paradigm
Carbohydrate ligands
Cellular expression
Structure
Biological roles of GBP-ligand interaction
CFG resources used in investigations
The best examples of CFG contributions to this paradigm are described below, with links to specific data sets. For a complete list of CFG data and resources relating to this paradigm, see the CFG database search results for P-selectin.
Glycan profiling
The glycans on the main target for P-selectin, P-selectin glycoprotein ligand 1 (PSGL-1), were analyzed.
Glycogene microarray
Regulation of P-selectin expression was analyzed under multiple conditions.
Knockout mouse lines
The phenotype of PSGL-1 knockout mice was analyzed by the CFG.
Glycan array
The comparative binding specificities of human and mouse selectins were analyzed.
Related GBPs
L-selectin and E-selectin
References
Acknowledgements
The CFG is grateful to the following PIs for their contributions to this wiki page: Kurt Drickamer, Yvette van Kooyk
