CARBOHYDRATE COMPOUNDS,
GLYCAN-BINDING PROTEINS & ANTIBODIES
Protein: hSiglec-2
Type: Ig-chimera (1-3d)
Buffer: 50 mM Tris, 100 mM glycine, pH7.0
Purity: Single band on Western blot
Storage temperature: -20ºC
Activity: ELISA assay with 0.25 µg Fc-chimera; works well with sialoside-SAAP probes
References:
Ig-chimera (1-3d): Brinkman-Van der
Linden EC, Sjoberg ER, Juneja LR, et al. Loss of N-glycolylneuraminic acid in
human evolution. Implications for sialic acid recognition by siglecs. J Biol Chem.
2000;275(12):8633-8640.
ELISA assay: Blixt O, Collins B,
Van Den Nieuwenhof I, et al. Sialoside secificity of the siglec family assessed
using novel multivalent probes: identification of potent inhibitors of myelin-associated
glycoprotein. J Biol Chem. 2003;278:31007-31019.
Glycan-binding Proteins
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