GENE MICROARRAY CORE (E)
Overview
This Core has developed a custom oligonucleotide microarray (Glyco-gene chip)
corresponding to human and murine glycan-binding protein (GBP) genes, glycosyltransferase
genes, and other genes relevant to this project. This Core is also screening gene expression
using mRNAs provided by investigators (upon Steering Committee approval) and the
Mouse Transgenics Core (F). All primary and processed data
obtained from the gene expression screening experiments are being deposited into the
Central Database. Details concerning
the genes represented and oligonucleotide sequences on the arrays is accessible to the
public on the Consortium website. As new GBP and glycosyltransferase gene sequences
become available they will be added to the chip. Glyco-gene chips are available for
distribution to investigators both within and outside of the Consortium upon approval
by the Steering Committee.
Specific Objectives
- Create a Glyco-gene chip with a unique set of human and murine probes containing all GBP,
glycosyltransferase, and other gene sequences relevant to this project. Approximately 1800
human and mouse gene targets are represented on the version 2 Glyco-gene chip.
- Update chips on a regular basis. As new GBP and glycosyltransferase gene sequences become
available they will be added to the chip.
- Coordinate with the Mouse Transgenics Core (F) to conduct gene
expression analysis on mRNA samples prepared from tissues of wild type and knockout mice.
- Conduct gene expression analysis for investigators (upon approval by the Steering Committee)
who prepare mRNA samples from tissues or cells and submit them for gene expression analysis.
- Provide Glyco-gene chips (upon approval by the Steering Committee) to other investigators within
and outside of the Consortium who wish to perform either the hybridization step or
complete analysis themselves.
Glyco-gene Chip Gene Lists
To access the gene list or perfect match target sequences for different versions of the Glyco-Gene chip, click below:
Core E Team
- Jim Paulson, Coordinator
- Steven Head, Director, TSRI DNA Array Core
- Gilberto Hernandez, Gene Chip Design, Sample Processing
- Tony Mondala, Staff Support, Liaison to Participating Investigators
- Lana Schaffer, Programmer, Statistician
- Suzanna Lee, Sample Processing
- Jen Hammond, Sample Processing
- Elizabeth Robison, Research Technician, Glycan Arrays
- Ryan McBride, Research Technician, Glycan Arrays
- Nina Tung, Administrative Assistant
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