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ANALYTICAL GLYCOTECHNOLOGY CORE (C) Glycan Profiling Results Glycan Analysis Timeline/Status Activities Overview This Core is conducting carbohydrate structure analysis of tissues, cells and glycan-binding protein (GBP) ligands. To this end, glycans and glycoconjugates are being isolated, fractionated and characterized by a variety of state-of-the-art analytical methods including mass spectrometric analysis using MALDI and FAB ionization methods, and ancillary methods such as ES-MS/MS to define the glycoforms present in cells and organs. This Core is working to achieve high-throughput mouse and human glycomics.  Information from this Core is being deposited into the Central Database and carbohydrate structures are being deposited into the Carbohydrate Database. Specific Objectives Determine carbohydrate structures elaborated by selected tissues that are thought to have ligands for GBPs from wild-type and glycosyltransferase knockout mice provided by the Mouse Transgenics Core (F). Determine carbohydrate structures of selected human tissues. Determine carbohydrate structures of selected cell lines supplied by the Protein-Glycan Interaction Core (H) or by Participating Investigators. Determine carbohydrate structures of glycoprotein and glycolipid ligands of specific GBPs which are identified by the Protein-Glycan Interaction Core (H) or by Participating Investigators. Core C Team Anne Dell, Coordinator Stuart Haslam, Director Lesley Harris, Administrative Assistant Simon North, Structural Analysis and Bioinformatics Jihye Jang Lee, Structural Analysis Anthony Etienne, Structural Analysis