PROTEIN-GLYCAN INTERACTION CORE (H)
Overview
This Core, in conjunction with the Glycan Array Synthesis Core (D), has developed two screening platforms for identifying lectin-ligand
interactions. Core H’s major objective is to conduct assays using the glycan arrays to determine the binding specificity of GBPs submitted by investigators through resource requests approved by the Steering Committee. The primary array platform is the printed microarray. Core H also maintains the first generation streptavidin/biotin-based glycan array for use as appropriate. A secondary
analysis method has been developed to quantitate the relative binding affinities of candidate
ligands. This Core is collaborating with the
Analytical Glycotechnology Core (C)
to isolate, biotinylate, and integrate natural glycans from glycoproteins, cells, and tissues into
the glycan array. Information on the protocols used to determine GBP binding specificity and the
raw and processed data from the analyses are being deposited into the
Central Database.
Specific Objectives
- Develop methods for screening the specificity of GBPs
- Coordinate with the Glycan Array Synthesis Core (D) to prioritize preparation of biotinylated oligosaccharides
- Establish the binding specificity and affinity of investigator-supplied GBPs approved by the Steering Committee
- Implement a secondary analysis method for quantitation of binding parameters
- Implement methods for isolating natural glycans for inclusion in the glycan array
Glycan-array platforms and generations
To view the current and previous versions of the glycan arrays click here.
Core H Team
- Rick Cummings, Coordinator
- David Smith, Director
- Jamie Heimburg-Molinaro, Analysis of Protein-Carbohydrate Interaction
- Jamie Heimburg-Molinaro, Administration
- Hong Ju, Analysis of Protein-Carbohydrate Interaction
- Yi Lasanajak, Analysis of Protein-Carbohydrate Interaction
- ChunMei Zhao, Analysis of Protein-Carbohydrate Interaction
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